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KMID : 1041219930350020120
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Journal of Sericultural and Entomological Science
1993 Volume.35 No. 2 p.120 ~ p.128
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Determination of Plasmids Encoding Crystal Toxic Protein Gene in Bacillus thuringiensis var kurstaki HD-1
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金哲永/Kim, Chul Young
金尙賢/金英勳/姜錫權/Kim, Sang Hyun/Kim, Young Hoon/Kang, Seok Kwon
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Abstract
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The objective of this study is to identify plasmids of Bacillus thuringiensis var. kurstaki HD-1 (B. t k HD-1) toxic to lepidopteran larvae. The results from agarose gel electrophoresis indicated that the bacterium contained 9 plasmids with approximate sizes of 1.4, 4.9, 5.4, 9.3, 10, 29, 44, 52, and 150 megadaltons(Md). By treating the wild type of B. t k HD-1 with either SDS or EtBr as curing agent 26 cured mutants of the bacterium were obtained, 9 of them were crystallifereous(cry+) and the others acrystallifereous(cry-). Plasmids from B. t k HD-1 were transferred to B. cereus 569 strR cry- recipients (Bc569 M1). Among 13 isolates of Bc569 M1 transcipient 11 of them were capable of producing the crystal toxic proteins. The plasmid patterns of Bc569 M1 transcipients and partially cured mutants of B. t k HD-1 on agarose gel electrophoresis suggested that the 29 and 44Md plasmids should be involved in the production of crystalline toxic proteins.
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